全文获取类型
收费全文 | 18860篇 |
免费 | 1574篇 |
国内免费 | 1853篇 |
出版年
2024年 | 27篇 |
2023年 | 285篇 |
2022年 | 429篇 |
2021年 | 660篇 |
2020年 | 570篇 |
2019年 | 873篇 |
2018年 | 817篇 |
2017年 | 500篇 |
2016年 | 541篇 |
2015年 | 718篇 |
2014年 | 1300篇 |
2013年 | 1402篇 |
2012年 | 923篇 |
2011年 | 1271篇 |
2010年 | 938篇 |
2009年 | 994篇 |
2008年 | 994篇 |
2007年 | 1123篇 |
2006年 | 969篇 |
2005年 | 856篇 |
2004年 | 720篇 |
2003年 | 595篇 |
2002年 | 578篇 |
2001年 | 370篇 |
2000年 | 354篇 |
1999年 | 301篇 |
1998年 | 321篇 |
1997年 | 236篇 |
1996年 | 243篇 |
1995年 | 285篇 |
1994年 | 220篇 |
1993年 | 198篇 |
1992年 | 191篇 |
1991年 | 170篇 |
1990年 | 142篇 |
1989年 | 116篇 |
1988年 | 113篇 |
1987年 | 94篇 |
1986年 | 62篇 |
1985年 | 107篇 |
1984年 | 155篇 |
1983年 | 120篇 |
1982年 | 137篇 |
1981年 | 59篇 |
1980年 | 52篇 |
1979年 | 51篇 |
1978年 | 40篇 |
1977年 | 19篇 |
1976年 | 13篇 |
1974年 | 11篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
51.
S. ESSEGHIR P. D. READY R. BEN-ISMAIL 《Biological journal of the Linnean Society. Linnean Society of London》2000,70(2):189-219
The phylogcny and mode of speciation of Mediterranean Phlebotomus of the subgenus Larroussius were inferred by comparative sequence analyses of a fragment of mitochondrial DNA (Cytochrome b) and of a nuclear gene (Elongation factor alpha). The molecular phytogenies were congruent basally, where their clades matched the species complexes defined by a few genitalic characters of each sex. Reticulate evolution was suggested for the most derived species complex [Phlebotomus perniciosus): the molecular phytogenies were incongruent, and mitochondrial-marker distribution was consistent with introgressive hybridizations not between sister species but between species whose ranges now overlap or abut. By considering the molecular phytogenies, the mitochondrial molecular clock and the ecological niches of the species, as well as the historical biogeography and palaeoecology of the Mediterranean subregion, we propose that the derived lineages arose from a sequential series of speciation events associated with habitat shifts promoted by progressive aridification. This 'taxon pulse'-like speciation occurred in the Pliocene, later than previously proposed in a vicariance hypothesis that invoked only tectonic events, but too early for Pleistocene Ice-age refugia to have played any role other than the isolation of geographical races. Speciation occurred before the proposed divergence of members of the Leishmania donovani complex and this helped to rule out any vector-parasite co-speciation or co-cladogenesis. 相似文献
52.
53.
《Saudi Journal of Biological Sciences》2022,29(2):831-839
The survival reduction after transplantation limited the clinical uses of stem cells so the current study explored preconditioning adipose-derived stem cells (ADMSCs) and all-trans retinoic acid (ATRA) effects on cisplatin that caused acute kidney injury (AKI). One hundred and fifty Sprague–Dawley male rats were distributed into five groups: control group; Cisplatin (CIS) group; CIS and ATRA group; CIS and ADMSC group, and CIS, ATRA, and ADMSCs group. Ten rats were euthanized after 3rd, 7th, and 11th days from CIS injection. Renal function, molecular studies, and histopathological analysis were studied. The preconditioning of ADMSCs with ATRA increased the viability of the cells which was reflected in the amelioration of kidney functions after CIS injection by the significant reduction of serum creatinine, microalbuminuria, as well as NO, and the significant rise of creatinine clearance, as well as SOD compared to the group of cisplatin. ATRA also supported ADMSCs by a significant down-regulation of caspase-3, il-6 and TGFβ1, and a significant up-regulation of HIF1, VEGF and CD31 compared to group of cisplatin which reversed the cisplatin effect. ATRA increased renoprotective properties of ADMSCs against cisplatin- induced AKI by reducing the apoptosis, inflammation, and stimulating angiogenesis. 相似文献
54.
《Journal of Plant Interactions》2013,8(2):91-99
Abstract The present research work focused on the metal translocation in the soil-plant system and subsequent metal stress on biochemical response of aquatic macrophytes growing along an industrial waste discharge channel. The bottom sediment of the effluent channel is highly contaminated with metals. High transfer factor (TF) for most of the metals indicated higher metal uptake by aquatic macrophytes of which Typha sp. was found to be the most suitable. Average TF was in the order of Fe (4.82) > Mn (3.91) > Cu (3.59) > Cd (2.29) > Zn (2.22) > Cr (1.83) > Pb (1.80). Hyper accumulation of metals within plants resulted in significant reductions in total chlorophyll, soluble sugar with an increase in protein and proline content. The investigation also demonstrated that exposure to high concentrations on metals resulted in enhanced activity of catalase (61.82–90.91%) and peroxidase (37.08–70.23%) in all examined macrophytes with a reduced (27.58–43.4%) or unchanged ascorbate peroxidase activity depending on plant species. 相似文献
55.
56.
57.
Chromium uptake bySaccharomyces cerevisiae and isolation of glucose tolerance factor from yeast biomass 总被引:2,自引:0,他引:2
Vlatka Gulan Zetic Vesna Stehlik-Tomas Slobodan Grba Lavoslav Lutilsky Damir Kozlek 《Journal of biosciences》2001,26(2):217-223
Fermentations with yeastSaccharomyces cerevisiae in semiaerobic and in static conditions with the addition of chromic chloride into the used molasses medium were analysed.
It was proved that the addition of optimal amounts of CrCl3 into the basal medium enhanced the kinetics of alcohol fermentations. The addition of 200 mg/l CrCl3 into the medium stimulated both the yeast growth and the ethanol production in all experimental conditions. On the other
hand, the results showed that Cr3+ ions were incorporated into yeast cells during fermentation. Under these conditions the accumulation of Cr3+ ions was performed by yeast cells during the exponential growth phase, and with enriched amounts of 30–45 (μg/gd.m. of cells.
Yeast biomass enriched with chromium ions was extracted with 01 mol/l NH4OH assuming that the extracts had the glucose tolerance factor (GTF). Then the extracts were passed through a gel-filtration
column in order to isolate and purify the GTF. The presence of GTF in the purified fractions was determined by measuring the
absorbance at 260 nm.
It is evident from the obtained results that the added purified fractions enhanced the rates of CO2 production as well as the glucose utilization during alcoholic fermentation. As expected, the enhancement of both rates depended
on the amounts of extracts added to the fermentation substrate. Thus, it is evident that purified extracts contained the GTF
compound, and that Cr3+ ions were bonded to the protein molecule. 相似文献
58.
59.
城市扩张过程中建设用地景观格局演变特征及其驱动力 总被引:3,自引:0,他引:3
剖析城市扩张过程中建设用地景观格局演变特征及其驱动力,不仅有助于城市生态环境问题的解决,而且为城市用地结构优化、城市景观规划等工作提供科学依据。以快速城市化的中型城市—扬州市为例,利用多期(1995、2000、2005、2010年和2015年)Landsat卫星影像、乡镇水平的扬州统计年鉴等数据,运用景观格局分析、增强回归树(Boosted regression trees)等方法,研究建设用地的扩张模式、形态及景观格局,定量探究地理、社会和经济因子对景观格局的影响机制,从而明晰景观格局演变特征及其驱动力。结果表明,1995—2015年,建设用地面积持续增加,填充式(Infilling)、边缘式(Edge-expansion)和跳跃式(Leapfrog)3种扩张模式在各时段均有出现,但其优势度随着城市发展而改变。建设用地的形态在城市扩张的过程中经历着"集聚"和"扩散"的交替变化过程,景观格局则出现了同质化倾向,景观破碎化下降、聚合度增加。地理因子(海拔和到县市中心的距离)对景观格局的综合影响虽然高于社会经济,但它的影响力却随着城市发展呈现出下降趋势,社会经济的作用则逐渐增强。海拔和人口密度的增加会促进景观的破碎化、抑制景观的集聚;人口数量的增加会促进景观的集聚、降低景观的破碎化;其余因子(到县市中心的距离、人均GDP和第二产业占比)的作用则随城市的发展而发生转变,如人均GDP对景观破碎化的作用表现为"促进→抑制"的转变、第二产业则为"抑制→促进"。 相似文献
60.